《营养科学》杂志：岩藻多糖抑制幽门螺杆菌与胃黏膜黏附机制

延伸阅读：

J Nutr Sci Vitaminol, 1999, 45, 325-336

Inhibitory Effect of Cladosiphon Fucoidan on the Adhesion of Helicobacter pylori to Human Gastric Cells

Abstract:

We studied the inhibitory effect of Cladosiphon fucoidan on the attachment of Helicobacter pylori (H. pylori), a gastroduodenal pathogen, to human gastric cell lines. The bacterial binding in these cell lines was inhibited more by Cladosiphon fucoidan (IC50=16-30mg/mL), than by the fucoidan from Fucus (IC50>30mg/mL). Dextran sulfate, another sulfated polysaccharide, did not inhibit the binding at all. Pre-incubating the bacterial suspension with fucoidans reinforced the inhibitory ability of these components, and reduced the IC50 value of Cladosiphon fucoidan to approximately 1mg/mL. However, the binding was not inhibited by pre-treatment of gastric cells with these com ponents. It was also shown that this fucoidan blocks both Leb and sulfatide-mediated attachment of H. pylori to gastric cells. Furthermore, fucoidan-binding proteins were found on the H. pylori cell surface by Western blot analysis. Thus, the inhibitory effect exerted by Cladosiphon fucoidan on binding between H. pylori and gastric cells might result from

the coating with this component of the bacterial surface.

First Authors:

Hideyuki SHIBATA

Correspondence:

Teruo YOKOKURA

All Authors:

Hideyuki SHIBATA, Itsuko KIMURA-TAKAGI, Masato NAGAOKA, Shusuke HASHIMOTO, Haruji SAWADA, Sadao UEYAMA and Teruo YOKOKURA